Cryo-EM reveals how a retrotransposon and CRISPR–Cas9 team up to insert genes
Cryo-electron microscopy structures of the zebra finch R2 retrotransposon, alone and bound to Cas9, reveal how target-primed reverse transcription initiates ...
Tag: CRISPR-Cas9
Rat model of normal pressure hydrocephalus shows multiregional blood-brain barrier leaks
A CRISPR-engineered rat carrying a truncating Cwh43 allele developed ventricular enlargement, impaired rotarod performance and multiregional blood-brain barrier permeability phenotypes ...
Molecular tools and consumer taste converge to reshape semi-arid tomato breeding
A new review synthesises how genetic diversity, molecular markers, genome editing and consumer sensory preferences can be integrated to breed ...
Fungal gene deletions fail to crack salicylic acid mystery in Trichoderma
Gene deletion experiments in Trichoderma virens show that the fungus makes salicylic acid through a pathway distinct from those used ...
Genes, Hormones and Smart Greenhouses: The New Science of Making Ornamental Plants Bloom on Demand
A new review in Botanical Studies examines how flowering time genes, plant hormones, gene editing and AI-driven greenhouse systems can ...
Molecular Tools Reshape Dengue Fight as Wolbachia and CRISPR Move From Lab to Field
A new review details how CRISPR gene editing, RNA interference, Wolbachia biocontrol and multi-omics surveillance are shifting dengue control from ...
Gene Behind Yellow-Green Leaves in Cauliflower Identified and Confirmed by CRISPR
Researchers fine-mapped and functionally validated CauYL, a magnesium chelatase H subunit gene essential for chlorophyll accumulation and normal leaf coloration ...
CRISPR snails: eyeless mutants made without a genome
Researchers generated eyeless F0 CRISPR-Cas9 pax6 mutants in the slipper limpet Crepidula fornicata using a de novo transcriptome instead of ...
Two CRISPR chd2 Knockout Zebrafish Strains Show Distinct Morphology and Behavior
Researchers generated the first two CRISPR chd2-knockout zebrafish strains and found that a four-base deletion and a one-base deletion in ...
PIK3CA mutation rewires immune and metabolic networks in cervical cancer cells
CRISPR-engineered cervical cancer cells carrying the PIK3CA-E545K mutation show coordinated immune signaling and metabolic rewiring, driven by suppressed lipid biosynthesis ...
CRISPR/Cas9 Disruption of oca2 and tyr Genes Alters Pigmentation in Zebrafish
Scientists used CRISPR/Cas9 to disrupt the tyr and oca2 pigmentation genes in zebrafish, reducing melanophores and visibly altering coloration in ...
Enzyme PLA2G4A fuels lung cancer growth through an HSF1 signaling axis
A new study reports that the enzyme PLA2G4A promotes non-small cell lung cancer proliferation, survival and metastasis through an SP1–IL-1β–MAPK–HSF1 ...
Lipid droplet kinase shapes lipid storage, stress response, and infectivity in Chagas parasite
Deleting the lipid droplet kinase gene in Trypanosoma cruzi reduced lipid droplet accumulation, increased resistance to hydrogen peroxide, and sharply ...
CRISPR centromere editing reshapes wheat chromosomes
A CRISPR–Cas9 strategy targeting centromeric repeats reconfigures up to 11 wheat chromosomes in one generation and yields heritable reciprocal translocations.
New Barcoded Yeast Library Maps Gene-Drug Interactions Across 262 Strains
A new CRISPR-Cas9 barcoded library, BarMix, profiles 262 diverse yeast strains against 33 compounds and reveals 281 chemogenomic interactions.
Scientists Challenge HERV-K102 Findings in Acute Myeloid Leukemia Study
A correspondence in Blood Research raises four methodological concerns about a study linking HERV-K102 envelope deletion to pyroptosis in acute ...
New mouse colorectal cancer cell line carries three key human mutations
Scientists created a mouse colorectal cancer cell line carrying Kras, Apc and Trp53 mutations that forms aggressive rectal tumors resembling ...
New mouse tool targets microvascular endothelial cells in muscle and fat
Scientists have created a CTRP9-CreERT2 knock-in mouse line whose tamoxifen-inducible reporter selectively labels microvascular endothelial cells in heart, skeletal muscle, ...
Azole-Resistant Aspergillus Emerges in Chinese Hospitals at 8.6 Percent
A surveillance study of 110 clinical Aspergillus isolates from two Chinese hospitals found 8.6 percent azole resistance in A. fumigatus ...
Lipid nanoparticles bring p53-targeted cancer therapy into focus
A new review argues that lipid nanoparticles, already clinically validated as mRNA carriers, could unify cancer therapy around restoring the ...
