Soft voting sharpens cryo-EM orientation recovery at low signal
A new Soft-Voting framework weights common-line evidence by confidence, lowering orientation errors on synthetic low-SNR data and yielding competitive ab ...
Tag: Cryo-EM
Cholesterol Binding Stabilizes THIK-1 Channel and Promotes Atherosclerosis
New research reveals that cholesterol binding stabilizes the activated state of the THIK-1 potassium channel, promoting atherosclerosis by enhancing inflammatory ...
Cellular chaperone EMC found essential for building calcium channels
Researchers report that the human EMC complex acts as a cotranslational chaperone for voltage-gated calcium channels, and that blocking this ...
Cryo-EM reveals how a retrotransposon and CRISPR–Cas9 team up to insert genes
Cryo-electron microscopy structures of the zebra finch R2 retrotransposon, alone and bound to Cas9, reveal how target-primed reverse transcription initiates ...
Scientists Solve Structure of the Transporter That Packs GABA and Glycine Into Brain Vesicles
Cryo-EM structures of human VIAAT reveal how the transporter recognizes both GABA and glycine and how chloride and proton binding ...
AI Takes Over Cryo-EM: Deep Neural Networks Now Automate Particle Picking, Map Reconstruction and Atomic Modelling
A comprehensive review details how deep neural networks are automating every stage of cryo-electron microscopy, from particle picking to high-resolution ...
Vitrobot-based protocol makes graphene oxide cryo-EM grids reproducible and accessible
A new Vitrobot-based protocol produces graphene oxide cryo-EM grids with 55 percent average coverage and supports apoferritin and β-galactosidase reconstructions ...
Cryo-EM reveals how unrelated phage peptides converge on the same bacterial target
A new cryo-EM structure shows how the phage lysis protein SglPP7 locks the essential transporter MurJ in an outward-facing state, ...
Cryo-EM freezes bacterial enzyme mid-action as it builds a cancer drug scaffold
Cryo-electron microscopy reveals the bacterial enzyme SacC, which builds the five-ring core shared by the anticancer drug trabectedin, stabilized by ...
Cargo proteins act as molecular bridges that drive AP-1 coat assembly
A new study shows that cargo peptides physically bridge neighboring AP-1 adaptor complexes through a µ1–σ1 interface, revealing that cargo ...
Supercomputers Catch GPCRs in Motion, Reshaping Drug Discovery
Enhanced sampling molecular dynamics simulations are revealing the hidden intermediate states, allosteric networks, and kinetic pathways that static structures cannot ...
Cryo-EM snapshots expose the molecular machine that keeps deadly tropical parasites alive
Researchers at the University of Liège and Rockefeller University have captured near-atomic-resolution cryo-EM structures of the trans-spliceosome, the essential RNA-processing ...
Hedgehog coronaviruses converge on aminopeptidase N as their entry receptor
Hedgehog merbecoviruses use aminopeptidase N as a functional entry receptor through a structurally distinct APN-binding mode, revealing convergent evolution and ...
Cell’s Insert and Undo Machines Caught Working Together in One ER Supercomplex
Cryo-EM structures reveal that the ER membrane protein complex and the Spf1 ATPase form a conserved supercomplex that juxtaposes membrane ...
First-in-class NVL inhibitors starve tumors by blocking ribosome assembly
Researchers have developed the first selective inhibitors of the NVL ATPase, blocking 60S ribosomal subunit assembly to activate p53 and ...
How HIV-1’s Vpr Protein Hijacks a Human Degradation Machine to Destroy Diverse Targets
New cryo-EM structures reveal how the HIV-1 protein Vpr reshapes the human CRL4(VprBP) E3 ligase to degrade host substrates of ...
Cryo-EM Exposes Hidden Architecture of a Bacterial Antiviral Weapon Found in Pathogens
Cryo-electron microscopy has revealed the first structure of a TenpIN type III toxin-antitoxin complex common to several pathogenic bacteria, showing ...
Lithium Blocks the Cellular Channels That Let Fungi Survive Fluoride
New research shows that lithium ions competitively block the sodium-dependent activation of eukaryotic fluoride exporter channels, constricting their pores and ...
H5N1 Variants From a Severe Human Case Show Weakened Grip on Host Receptors
Cryo-EM and binding assays reveal that hemagglutinin mutations E190D and Q226H in H5N1 viruses from a severely ill British Columbia ...
Cryo-EM Captures the Step-by-Step Activation of the MERS-CoV Spike Protein
Time-course cryo-electron microscopy has revealed the sequence of structural changes by which DPP4 receptor binding progressively activates the MERS-CoV spike ...
