A new, updated account of poroid fungi in North America
Poroid fungi, commonly known as "polypores," are among the most frequently encountered fungi throughout the year because of their large ...
![\"A](\"https:\/\/i0.wp.com\/bioengineer.org\/wp-content\/uploads\/2024\/06\/Enzymes-instead-of-cyanide-Researchers-develop-biocatalytic-process-for-nitrile.jpeg?ssl=1\")
<\/p>\nIf the household cleaner emits a lemon-like odour, this may be due to a nitrile called citronellyl nitrile. These versatile chemical nitrile groups are also used in the manufacture of active pharmaceutical ingredients, superglue and chemical-resistant gloves. The prevalent production process used so far has required a chemical reaction of certain molecules with highly toxic cyanide. Margit Winkler from the Institute of Molecular Biotechnology at TU Graz, together with Ludmila Mart\u00ednkov\u00e1 from the Institute of Microbiology at the Czech Academy of Sciences, has now developed a biocatalytic process that does not require cyanide, works at room temperature and therefore requires less energy and produces less harmful waste. The project was funded by the Austrian Science Fund FWF and the Czech Science Foundation (GACR).<\/p>\n
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Credit: IMBT – TU Graz<\/p>\n
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If the household cleaner emits a lemon-like odour, this may be due to a nitrile called citronellyl nitrile. These versatile chemical nitrile groups are also used in the manufacture of active pharmaceutical ingredients, superglue and chemical-resistant gloves. The prevalent production process used so far has required a chemical reaction of certain molecules with highly toxic cyanide. Margit Winkler from the Institute of Molecular Biotechnology at TU Graz, together with Ludmila Mart\u00ednkov\u00e1 from the Institute of Microbiology at the Czech Academy of Sciences, has now developed a biocatalytic process that does not require cyanide, works at room temperature and therefore requires less energy and produces less harmful waste. The project was funded by the Austrian Science Fund FWF and the Czech Science Foundation (GACR).<\/p>\n
To replace the cyanide, the research group uses enzymes and combines three individual reactions into a cascade. The starting materials are carboxylic acids, such as fatty acids from plant oils or lignin, which is a by-product of wood processing. In the first step, the carboxylic acid is converted to an aldehyde by adding sugar, oxygen and the enzyme carboxylate reductase contained in living bacterial cells. However, this aldehyde is highly reactive and therefore unstable, and in many cases also volatile. To capture this substance, the aldehyde is allowed to react with hydroxylamine, which converts it into oxime, which is stable.<\/p>\n
Oxime is a \u2018watered\u2019 form of nitrile, which is why the enzyme aldoxime dehydratase is used in the final step. This enzyme is also confined in bacterial cells and is responsible for water abstraction from the oxime. Once this has been done, the cascade has achieved its objective and the desired nitrile has been produced.<\/p>\n
This method can now be used for the preparation of small nitrile quantities. This is already a promising technology for very potent fragrances, for example, of which only a small amount is needed to achieve an effect. Optimisation work is still required for the production of larger quantities of nitrile in order to be able to use the biocatalytic process at reasonable costs. Currently, highly diluted solutions are used in the first process step and a large reactor would be required to produce large quantities, which is not economic yet. Margit Winkler is therefore already conducting research on how to make the first step more efficient. Dehydration of the oxime alone, on the other hand, is extremely efficient and already reached technical maturity for production today.<\/p>\n
\u201cI\u2019ve been interested in cyanide chemistry ever since I wrote my master thesis, and as we know from Agatha Christie\u2019s novels, cyanide is highly toxic,\u201d says Margit Winkler. \u201cIt was therefore important for us to find a way to avoid this hazardous substance in production. The fatty acids we use come from vegetable oils. So, we really do have a bio-based production method here. We would now like to improve it even further so that it can be used widely.\u201d<\/p>\n
This research area is anchored in the Field of Expertise \u201cHuman & Biotechnology<\/em>\u201c, one of the five strategic research areas of TU Graz.<\/em><\/p>\n Journal of Biotechnology<\/p>\n<\/p><\/div>\n 10.1016\/j.jbiotec.2024.02.005 <\/i><\/p>\n<\/p><\/div>\n Experimental study<\/p>\n<\/p><\/div>\n Not applicable<\/p>\n<\/p><\/div>\n Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry<\/p>\n<\/p><\/div>\n 20-Mar-2024<\/p>\n<\/p><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":" If the household cleaner emits a lemon-like odour, this may be due to a nitrile called citronellyl nitrile. These versatile chemical nitrile groups are also used in the manufacture of active pharmaceutical ingredients, superglue and chemical-resistant gloves. The prevalent production process used so far has required a chemical reaction of certain molecules with highly toxic […]<\/p>\n","protected":false},"author":8,"featured_media":228588,"comment_status":"","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"jnews-multi-image_gallery":[],"jnews_single_post":[],"jnews_primary_category":[],"jnews_override_bookmark_settings":[],"jnews_social_meta":[],"jnews_review":[],"enable_review":"","type":"","name":"","summary":"","brand":"","sku":"","good":[],"bad":[],"score_override":"","override_value":"","rating":[],"price":[],"jnews_override_counter":[],"footnotes":""},"categories":[185],"tags":[],"class_list":["post-228587","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-news"],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/bioengineer.org\/wp-content\/uploads\/2024\/06\/Enzymes-instead-of-cyanide-Researchers-develop-biocatalytic-process-for-nitrile.jpeg?fit=700%2C350&ssl=1","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/posts\/228587","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/comments?post=228587"}],"version-history":[{"count":0,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/posts\/228587\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/media\/228588"}],"wp:attachment":[{"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/media?parent=228587"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/categories?post=228587"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bioengineer.org\/wp-json\/wp\/v2\/tags?post=228587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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A new, updated account of poroid fungi in North America
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