Credit: RUDN University
RUDN University chemists have developed a simple and convenient method for producing derivatives of the natural polymer chitosan. These derivatives are non-toxic and have a pronounced antibacterial activity at the level of modern antibiotics. These substances can be used in the production of antibacterial protective films for food. The article is published in the journal Food Chemistry.
Preservatives are widely used in the food industry. They are necessary to extend the shelf life of products. On the other hand, preservatives reduce food quality. Some of them can cause allergies (benzoic acid) or be toxic (nitrates, nitrites). Synthetic waxy substances used to coat fruits can be carcinogenic (biphenyl is prohibited in the US and EU). This explains the importance of finding new preservatives that are effective and safe.
Chitosan is a natural polymer derived from chitin, the main component of insect and crustacean shells. It has antibacterial properties and is already used in the food industry for packaging and coating products with a protective film. However, its activity is much inferior to antibiotics.
To obtain non-toxic and highly active derivatives of chitosan, RUDN University chemists Andreii Kritchenkov and Margarita Kurasova and their colleagues have modified chitosan with organic azides by “sewing” them to the polymer chain. Azides are derivatives of hydrazoic acid HN3. Both organic and inorganic azides are toxic, but their compounds with chitosan are not poisonous. Antibacterial activity of the obtained substances, azido chitosan, was significantly higher than that of chitosan.
The experiment showed that samples of polymers based on chitosan had antibacterial activity at the level of antibiotics. The researchers compared this parameter for azido chitosan with the two most common antibiotics – ampicillin and gentamicin.
Antibacterial activity was determined by diffusion into the agar (nutrient) layer. A disk of filter paper impregnated with the test substance (or comparison antibiotic) was placed on the agar layer sown with a bacterial culture. The substance penetrates the agar, killing bacteria or slowing their reproduction. As a result, a growth suppression zone is formed around the disk, which differs from the rest of the agar surface visually. The value of an antibacterial activity is estimated by the diameter of the formed zones. Toxicity was determined by a standard colorimetric test.
An obtained derivative of chitosan – azido chitosan – cleaned Staphylococcus aureus from a zone of 26 mm in diameter (the indicator for ampicillin was 30 mm), and of 18 mm for Escherichia coli (in gentamycin it was 22 mm).
That is, the polymer has almost the same antibacterial activity as antibiotics. This is extremely important, because the use of antibiotics as components of antibacterial films is highly unacceptable because it leads to the emergence of resistant strains of bacteria. Chitosan compounds do not have this disadvantage.
New chitosan derivatives can be used as harmless and effective preservatives to create protective films for food on their basis.
Novel non-toxic high efficient antibacterial azido chitosan derivatives with potential application in food coatings.
15 December 2019, Volume 301, 125247
Research area: chemistry
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