• HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
Thursday, October 2, 2025
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News Chemistry

Researchers explain signals of CpG ‘traffic lights’ in DNA

Bioengineer by Bioengineer
April 9, 2019
in Chemistry
Reading Time: 4 mins read
0
IMAGE
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram

IMAGE

Credit: @tsarcyanide/MIPT Press Office

A research team featuring bioinformaticians from the Moscow Institute of Physics and Technology (MIPT) has identified reliable markers of gene activity. The discovery has potential for future applications in clinical practice. The findings are reported in BMC Genomics.

In terms of macromolecular chemistry, DNA is a polymer, or polynucleotide, composed of four kinds of repeated units known as nucleotides. What makes the four types different are the associated nitrogenous bases: adenine (A), thymine (T), guanine (G), and cytosine (C). A DNA region with a C followed by G, connected via a phosphate (p), is known as a CpG dinucleotide (figure 1).

Genes are DNA regions carrying primary information about the RNA and proteins produced by a cell. The DNA sequence is usually the same across all cells in an organism, but the “working” genes actually involved in RNA synthesis are different for every cell type. This is enabled by regulatory mechanisms functioning as switches that launch or halt the production of specific RNAs. Once a certain level of organism complexity is reached in evolution, the number of genes does not increase any longer. Instead, more elaborate regulatory programs evolve to make sure the right genes are activated at the right time.

As of now, two major types of mechanisms regulating gene transcription (RNA synthesis) have been studied in detail: those involving so-called transcription factors and epigenetic regulators. Transcription factors are regulatory proteins capable of binding to DNA by recognizing certain nucleotide sequences in the regulatory regions of genes. Once a transcription factor has bound to DNA, it engages special cell machinery to initiate RNA synthesis from that particular gene. A large number of such factors is known (over 1,500 in humans). Their combinations regulate how active transcription should be and whether it should happen at all.

Epigenetic regulation involves mechanisms that control how active a gene is without affecting the primary DNA structure. One of such mechanisms is DNA methylation. It is mostly achieved by attaching a methyl group (CH?) to the cytosine in a CpG dinucleotide (figure 2). Once attached, the methyl group serves as a marker signaling which genes are active or repressed, ultimately determining the cell type. It is no surprise that DNA methylation is associated with numerous biological processes, both normal and pathological. Abnormal DNA methylation is observed in cancer, metabolic disorders, cardiovascular, neurodegenerative, and other diseases.

By binding to cytosine nucleotides in distinct functional regions of the DNA, a methyl group can have distinct effects. For example, methylation of regions close to transcription start sites tends to suppress gene activity. Conversely, methylation of cytosines inside the gene usually serves to activate it (figure 3).

“In our previous papers, we showed that methylation of certain CpG dinucleotides was strongly associated with gene activity. We called such dinucleotides CpG traffic lights. Now we have demonstrated that the methylation of CpG traffic lights is a better indicator of gene activity than promoter or gene body methylation. In addition, we’ve shown that enhancers — DNA regions located away from genes but regulating their activity — are enriched in CpG traffic lights,” explained Yulia Medvedeva, the senior author of the paper and associate professor of bioinformatics and systems biology at MIPT, who leads the regulatory transcriptomics and epigenomics group at the Research Center of Biotechnology of the Russian Academy of Sciences.

“We noted that CpG traffic lights are conserved in the course of evolution. That is, these positions are relatively less susceptible to mutations, supporting their functional significance,” added Anna Lioznova, the first author of the study and a doctoral student at the Research Center of Biotechnology, RAS.

“We used to think that the main role of CpG traffic lights was to switch the regions of transcription factor binding from the active to the passive state,” said study co-author Ivan Kulakovskiy, a researcher at Engelhardt Institute of Molecular Biology and the Institute of Mathematical Problems of Biology, RAS. “Surprisingly, that previously explained and described mechanism proved to account for a relatively small share in the overall number of traffic lights. We suppose that the operation of traffic lights is tied to what’s known as an activity ‘map’ of DNA regions [aka. chromatin states], but the specific mechanisms are still to be discovered.”

CpG traffic lights are CpG dinucleotides whose methylation reflects the activation or repression of a gene encoded nearby. In other words, such dinucleotides signal whether RNA is to be synthesized from this gene, and ultimately whether the associated protein will be produced. By studying CpG traffic lights, the researchers hope to understand the mechanisms of gene regulation. In clinical practice, determining the status of cytosine methylation produces more reliable results, compared with direct measurements of gene activity. This opens up prospects for the clinical use of CpG traffic lights as effective indicators of gene activity.

###

The study was conducted by researchers from MIPT, Lomonosov Moscow State University, King Abdullah University of Science and Technology in Saudi Arabia, and the following institutions of the Russian Academy of Sciences: Research Center of Biotechnology, Vavilov Institute of General Genetics, the Institute of Mathematical Problems of Biology, Engelhardt Institute of Molecular Biology, and the Institute for Information Transmission Problems.

This research was supported by the Russian Foundation for Basic Research and the Russian Science Foundation.

Media Contact
Varvara Bogomolova
[email protected]

Original Source

https://mipt.ru/english/news/researchers_explain_signals_of_cpg_traffic_lights_in_dna

Related Journal Article

http://dx.doi.org/10.1186/s12864-018-5387-1

Tags: BiochemistryBioinformaticsBiologyGenesGenetics
Share12Tweet8Share2ShareShareShare2

Related Posts

Innovative PtCu@Zeolite Propane Dehydrogenation Catalyst Developed via Ion Exchange and Displacement Reaction Strategy

October 2, 2025
blank

Nanoreactor Cage Harnesses Visible Light for Ultra-Selective Catalytic Cross-Cycloadditions

October 2, 2025

Palladium Filters Pave the Way for More Affordable, Efficient Hydrogen Fuel Production

October 1, 2025

Revolutionary Organic Molecule Poised to Transform Solar Energy Harvesting

October 1, 2025
Please login to join discussion

POPULAR NEWS

  • New Study Reveals the Science Behind Exercise and Weight Loss

    New Study Reveals the Science Behind Exercise and Weight Loss

    91 shares
    Share 36 Tweet 23
  • New Study Indicates Children’s Risk of Long COVID Could Double Following a Second Infection – The Lancet Infectious Diseases

    78 shares
    Share 31 Tweet 20
  • Physicists Develop Visible Time Crystal for the First Time

    74 shares
    Share 30 Tweet 19
  • How Donor Human Milk Storage Impacts Gut Health in Preemies

    64 shares
    Share 26 Tweet 16

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Recent News

Barriers Facing Roma Women in Primary Healthcare

Innovative PtCu@Zeolite Propane Dehydrogenation Catalyst Developed via Ion Exchange and Displacement Reaction Strategy

Revolutionary All-Optical Neuron Powers Nonlinear Computing

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 60 other subscribers
  • Contact Us

Bioengineer.org © Copyright 2023 All Rights Reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In
No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

Bioengineer.org © Copyright 2023 All Rights Reserved.