• HOME
  • NEWS
    • BIOENGINEERING
    • SCIENCE NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • FORUM
    • INSTAGRAM
    • TWITTER
  • CONTACT US
Saturday, August 20, 2022
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
    • BIOENGINEERING
    • SCIENCE NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • FORUM
    • INSTAGRAM
    • TWITTER
  • CONTACT US
  • HOME
  • NEWS
    • BIOENGINEERING
    • SCIENCE NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • FORUM
    • INSTAGRAM
    • TWITTER
  • CONTACT US
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News

The missing links: Finding function in lincRNAs

Bioengineer by Bioengineer
July 19, 2022
in Science News
0
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram

Genomes contain regions between protein-coding genes that produce lengthy RNA molecules that never give rise to a protein. These long intergenic non-coding RNAs (lincRNAs) are thought to have important functions, such as regulating responses to environmental change. However, a paucity of well-annotated lincRNA data, especially for crop plants, has precluded a deeper understanding of their roles.

Kyle Palos

Credit: Image credit: Boyce Thompson Institute.

Genomes contain regions between protein-coding genes that produce lengthy RNA molecules that never give rise to a protein. These long intergenic non-coding RNAs (lincRNAs) are thought to have important functions, such as regulating responses to environmental change. However, a paucity of well-annotated lincRNA data, especially for crop plants, has precluded a deeper understanding of their roles.

Up until now, there have been no systematic genome-wide studies that both confirmed DNA sequences that produce lincRNAs and proposed functions for those lincRNAs. Plus, data are reported differently across studies, making direct comparisons among them difficult.

These barriers inspired researchers at the Boyce Thompson Institute to take a comprehensive look at the identity, production and function of lincRNAs in four species in the mustard family, including the model organism Arabidopsis thaliana, and Brassica rapa, a species that produces boy choy, turnips and other food crops.

The group found locations across all four genomes that encoded lincRNAs, proposed functions for them, and confirmed the function of some lincRNAs involved in germination in Arabidopsis, creating an approach that could help researchers further understand the enigmatic molecules in all species, from crops to humans.

Their results were published in The Plant Cell on June 6.

“Our goal was to generate extensive and more actionable data for researchers to understand lincRNA function,” said Kyle Palos, first author on the study and a postdoctoral fellow in the lab of BTI Assistant Professor Andrew Nelson.

“This project started small and mushroomed after we realized we couldn’t begin to figure out lincRNA function without having thoroughly annotated genomes to know what lincRNAs were even present,” said Nelson, who is the corresponding author of the paper. “Kyle really led the charge on everything that went into this paper.”

The team hypothesized that lincRNA production and function are limited to certain cell types and environmental conditions. The more common data sets don’t cover that level of detail, “so it’s easy to miss a lot due to limited sampling,” Palos said. “Our comprehensive approach merges a high-throughput, top-level analysis that identifies lincRNAs with a deeper dive into their likely functions, to give the full picture.”

The study utilized a unified approach to gathering and annotating lincRNA data that could be easily adopted by other groups. According to Palos, this would facilitate comparisons across different experiments and species as the body of plant lincRNA data continues to grow.

The team uploaded its results to CyVerse, a free and open-science workspace where researchers can store, access and analyze data all in one place.

“We made it as simple as possible for others to search our results for lincRNAs involved in a plant trait or pathway of interest, and in responses to temperature and other environmental stressors,” Palos said.

The team’s methods could also help resolve long-standing questions with genome-wide association studies (GWAS) that identify correlations between plant traits and gene variants: What is going on with the variants that fall outside of protein-coding regions? Are these variants within other genes (i.e., lincRNAs) or regulatory elements?

“You need a properly annotated genome to know that, before you can determine the variant’s effect and how to modulate it to produce your crop of choice,” said Nelson, who is also an adjunct assistant professor in the School of Integrative Plant Science at Cornell University.

In the study, the team processed over 20,000 publicly available RNA sequence data sets from the four mustard species, supplemented with its own sequencing data, to identify thousands of lincRNAs, and then annotated the lincRNAs with genomic, structural and other information.

They assigned putative functions to the lincRNAs based on their similar expression patterns to protein-coding genes with known functions. Next, the team deleted a subset of lincRNAs that appeared to play roles in seed germination and development in Arabidopsis, which led to reductions in germination, thus validating their approach to determine lincRNA function.

In addition to ongoing studies of the germination-related lincRNAs, the team is applying its methods to lincRNAs in four more important crops for which a wealth of RNA sequence data is available – rice, maize, sorghum and Setaria italica (foxtail millet) – and has plans to expand into another nine well-sequenced species.

“Plant genome research often falls behind mammalian research, but with lincRNAs, we’re still very much in the dark across all species,” Nelson said. “Researching lincRNA in plants could have an impact on human health and crops alike, by helping us understand their fundamental properties, regardless of the species.”

Co-authors of the paper include BTI Assistant Professor Aleksandra Skirycz.

About Boyce Thompson Institute:

Opened in 1924, Boyce Thompson Institute is a premier life sciences research institution located in Ithaca, New York. BTI scientists conduct investigations into fundamental plant and life sciences research with the goals of increasing food security, improving environmental sustainability in agriculture, and making basic discoveries that will enhance human health. Throughout this work, BTI is committed to inspiring and educating students and to providing advanced training for the next generation of scientists. BTI is an independent nonprofit research institute that is also affiliated with Cornell University. For more information, please visit BTIscience.org.

###



Journal

The Plant Cell

DOI

10.1093/plcell/koac166

Method of Research

Experimental study

Subject of Research

Not applicable

Article Title

Identification and functional annotation of long intergenic non-coding RNAs in Brassicaceae

Article Publication Date

6-Jun-2022

COI Statement

None cited.

Share12Tweet7Share2ShareShareShare1

Related Posts

Tweezers of sound lift objects stably and without contact.

Scientists fine-tune “tweezers of sound” for contactless manipulation of objects

August 20, 2022
How to regenerate mouse hearts

Harnessing the heart regeneration ability of marsupials

August 20, 2022

UTSW Clinical trial sets stage for new paradigm in kidney cancer treatment

August 19, 2022

Study: New model for predicting belief change

August 19, 2022

POPULAR NEWS

  • Picture of the horse specimen.

    Ancient DNA clarifies the early history of American colonial horses

    57 shares
    Share 23 Tweet 14
  • Fatigue, headache among top lingering symptoms months after COVID

    40 shares
    Share 16 Tweet 10
  • Chi-Huey Wong awarded Tetrahedron Prize for Creativity in Organic Synthesis

    38 shares
    Share 15 Tweet 10
  • Dogs lying in the middle of the road after sunrise at Kewa Pueblo, in no hurry to start the day

    35 shares
    Share 14 Tweet 9

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Tags

VirusWeather/StormsWeaponryUniversity of WashingtonZoology/Veterinary ScienceUrogenital SystemVaccineVehiclesVirologyVaccinesUrbanizationViolence/Criminals

Recent Posts

  • Scientists fine-tune “tweezers of sound” for contactless manipulation of objects
  • Harnessing the heart regeneration ability of marsupials
  • UTSW Clinical trial sets stage for new paradigm in kidney cancer treatment
  • Study: New model for predicting belief change
  • Contact Us

© 2019 Bioengineer.org - Biotechnology news by Science Magazine - Scienmag.

No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

© 2019 Bioengineer.org - Biotechnology news by Science Magazine - Scienmag.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In