A plant's defense systems help each other. When one system fails, another one can – at least in part – take over.
Researchers at the Norwegian University of Science and Technology 's (NTNU) Department of Biology have been collaborating with colleagues from Imperial College London and The Sainsbury Laboratory to discover more about how plants defend themselves. Their results have been published in the 26 June 2018 issue of Science Signaling.
Identifying the danger
A plant's cell walls function as an outer skeleton that protects them against various threats. This structure is vital, and therefore plants have developed mechanisms that monitor the cell walls and detect when they are being damaged.
When a cell wall sustains damage, the plant will normally try to minimize the damage and repair it. The goal is to restore the plant's normal state, or equilibrium.
The plant needs to respond differently depending on the type of danger threatening it. Initially, the plant is not able to discern whether the damage to the cell wall is being caused by drought or a disease, for example.
So how can plants identify the danger and provide the right response?
Need numerous types of defense
Associate Professor Thorsten Hamann at NTNU has been central to finding the answer to this question.
"Drought requires a plant to adjust its metabolism, whereas disease requires the plant to activate various immune responses," explains Hamann.
Different chemical processes are involved depending on the threat that the plant has to respond to. Physical damage to the cell wall demands a completely different solution from the plant than disease does.
The researchers exposed thale cress (Arabidopsis thaliana) to various injuries to see how the plants would react.
Thale cress is a fairly common vascular plant in the Brassicaceae family. It is usually an annual, fairly easy to grow, and the 30 000 genes of the species are fully mapped.
These qualities have led to the species being used as a "model organism" by researchers to provide insight into biological processes. Their hope is that the results will prove valid for more species than just the trial species itself. In this way the researchers can learn something about several species by examining one species.
Attacking the plants
The plant's cell walls consist of proteins and assorted sugars (carbohydrates), such as cellulose, pectin and lignin.
The researchers attacked some of the plants with enzymes that break down the cell walls. Other plants had a substance added that inhibits the formation of cellulose. The researchers then investigated the plants' chemical responses.
They disconnected 27 different genes to observe the effects. Five of the genes were important in maintaining the equilibrium of the cell walls. The experiments provided a basis for identifying multiple enzymes (kinases) and channel proteins involved in the plant's defence mechanisms. A number of genes are involved in producing these substances.
Double defense duty
The most interesting finding seems to be that two defence systems can act as a kind of backup for each other.
"We found that if we blocked the plants' immune response, the mechanisms that maintain balance in the cell walls could partially compensate for this blockage. They became a kind of reserve defence system," says Hamann.
The article in Science Signaling provides a considerably improved understanding of relationships where external influences trigger specific reactions in the plants.
"We can see how different physical influences trigger different specific chemical responses," says Hamann.
Knowing that, it's easy to see that humans can influence plants to react in certain ways.
Important for agriculture
The results also have practical utility in addition to being interesting basic research.
Knowing more about the various defence mechanisms in plants opens up new possibilities for solutions that may help plants' ability to resist different threats more effectively.
The results of the thale cress experiments could play an important role for agriculture, such as in cultivating rice and corn strains that produce better crops. Annually, more than one billion tonnes of maize are produced, and rice production is around 750 million tonnes. Many people and livestock rely on these plants as a central part of their diet.
Large populations stand to experience especially tangible value from this research.
Reference: The plant cell wall integrity maintenance and immune signaling systems cooperate to control stress responses in Arabidopsis thaliana. Sci. Signal. 26 Jun 2018: Vol. 11, Issue 536, eaao3070 DOI: 10.1126/scisignal.aao3070 http://stke.sciencemag.org/content/11/536/eaao3070
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