• HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
Tuesday, October 28, 2025
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News Biology

Old methods prove true for studying proteins

Bioengineer by Bioengineer
October 19, 2020
in Biology
Reading Time: 2 mins read
0
IMAGE
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram

IMAGE

Credit: © 2020 ?ukasz Jaremko and Vladlena Kharchenko

A fresh new look at an old technique in protein biochemistry has shown that it should be reintroduced to the spectroscopy toolkit.

For decades, scientists have used nuclear magnetic resonance (NMR) spectroscopy to probe the molecular motions of proteins on various timescales. This technique has revealed aspects of enzyme reactions, protein folding and other biological processes, all on an atomic scale.

Typically, spectroscopists will gauge the rotation of NMR-active atoms in the protein backbone with and without proton irradiation to calculate a ratio known as a steady-state nuclear Overhauser effect (NOE); however, it was not always done this way.

Before steady-state NOE experiments became the norm in biological investigations, scientists would often take a greater number of measurements over the course of an irradiation experiment. This method, termed “dynamic” NOE, might seem more complicated, but according to Ph.D. student Vladlena Kharchenko, it is no more time consuming than steady-state NOE, while dynamic NOE provides additional information about protein flexibility and is far more accurate to minute biological motions in proteins.

“It works for proteins and makes studying their dynamics even more accurate,” says Kharchenko, a member of ?ukasz Jaremko’s lab at KAUST. “Our message to biological NMR spectroscopists is simple: ‘Don’t be afraid of dynamic NOE.'”

To prove the technique’s worth, Kharchenko, Jaremko and their team performed a series of NMR experiments on ubiquitin, a globular protein that regulates a range of processes inside the cell. Working with Mariusz Jaremko, also from KAUST, and collaborators in Poland, the researchers collected both steady-state and dynamic NOE measurements and demonstrated that the dynamic approach is always preferable–except under a few specific conditions, such as when instrument access is limited or when proteins degrade very rapidly.

Notably, the steady-state approach proved especially prone to errors in regions of the ubiquitin protein that were flexible and disposed to moving around. The dynamic technique, in comparison, offered no such misleading results.

In light of their findings, the KAUST team hopes that other scientists with an interest in atomic-level protein mechanics will now begin to adopt, or at least reconsider, dynamic NMR methods. Kharchenko says that sometimes, “it’s worth dusting off forgotten methods and checking if they fit to new emerging questions and systems of research interest.”

###

Media Contact
KAUST Discovery team
[email protected]

Original Source

https://discovery.kaust.edu.sa/en/article/1037/old-methods-prove-true-for-studying-proteins

Related Journal Article

http://dx.doi.org/10.1007/s10858-020-00346-6

Tags: Atomic/Molecular/Particle PhysicsBiochemistryBiologyCell BiologyElectromagneticsMolecular BiologyMolecular PhysicsNuclear Physics
Share12Tweet8Share2ShareShareShare2

Related Posts

blank

Vitamin D Deficiency Linked to Higher Risk of Complicated Deliveries

October 28, 2025
New Steinernema abbasi Isolate Controls Crop Pests

New Steinernema abbasi Isolate Controls Crop Pests

October 28, 2025

Scientists Discover New Genetic Cause of Microcephaly

October 28, 2025

Ovine Toxoplasmosis Risks Uncovered in Iraq Study

October 28, 2025
Please login to join discussion

POPULAR NEWS

  • Sperm MicroRNAs: Crucial Mediators of Paternal Exercise Capacity Transmission

    1288 shares
    Share 514 Tweet 322
  • Stinkbug Leg Organ Hosts Symbiotic Fungi That Protect Eggs from Parasitic Wasps

    310 shares
    Share 124 Tweet 78
  • ESMO 2025: mRNA COVID Vaccines Enhance Efficacy of Cancer Immunotherapy

    198 shares
    Share 79 Tweet 50
  • New Study Suggests ALS and MS May Stem from Common Environmental Factor

    135 shares
    Share 54 Tweet 34

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Recent News

Post-COVID Nasal Cells Altered by TNFα, TGFβ

Advanced ZIF-90/Nb2C Nanohybrids Boost Energy Storage

Revolutionary Phosphate Strategy Yields High-Performance LiMnPO4

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 67 other subscribers
  • Contact Us

Bioengineer.org © Copyright 2023 All Rights Reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In
No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

Bioengineer.org © Copyright 2023 All Rights Reserved.