• HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
Wednesday, March 29, 2023
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News

Microseeding: A new way to overcome hemihedral twinning?

Bioengineer by Bioengineer
December 15, 2016
in Science News
Reading Time: 2 mins read
0
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram
IMAGE

Credit: Harada et al.

Twinning is a crystal-growth disorder in which the specimen is composed of distinct domains whose orientations differ but are related in a particular, well-defined way. Twinning, which is a known problem in protein crystallography, usually hampers high-quality crystal structure determination unless it is detected and either avoided or corrected. Although effective computational methods have been developed for the determination of structures using data from twinned crystals (known as 'detwinning'), if possible it is preferable to obtain crystals that are not twinned.In some cases, optimising the length of the protein fragment used for crystallisation can lead to the growth of untwinned crystals, but this is a time-consuming process.

In a recent paper in Acta Crystallographica Section F, microseeding was used to produce untwinned crystals of LigM, an O-demethylase from Sphingobium sp. SYK-6, using twinned crystals as seeds. Microseeding is one of several seeding techniques that are used to successfully separate nucleation events from crystal-growth events. In this technique, crystals are used as seeds and introduced into new drops which are equilibrated at lower levels of supersaturation. It has frequently been used to improve reproducibility in crystallization and can yield different crystal forms.

In the work described by Harada et al. [(2016). Acta Cryst. F72, 897-902; doi:10.1107/S2053230X16018665], around 50% of the initially obtained crystals of LigM were hemihedrally twinned. These crystals were then used as a seed stock for microseeding. In combination with optimization of the reservoir solution, this led to crystals that were not twinned and belonged to a different space group. It is hoped that this method will have potential as a more general simple method for overcoming hemihedral twinning in protein crystals.

###

Media Contact

Dr. Jonathan Agbenyega
[email protected]
024-434-2878
@iucr

http://www.iucr.org

############

Story Source: Materials provided by Scienmag

Share12Tweet8Share2ShareShareShare2

Related Posts

Understanding protein variants could improve the diagnosis and treatment of lung cancer

Seer researchers find undiscovered links between lung cancer and multiple distinct protein variants via deep, unbiased proteomics analysis

March 29, 2023
Ochsner Gallup

Ochsner Health named 2023 Gallup Exceptional Workplace Award Winner

March 29, 2023

New chip design to provide greatest precision in memory to date

March 29, 2023

We are not yet approaching any maximum human lifespan, according to an examination of human mortality over time and across 19 countries

March 29, 2023
Please login to join discussion

POPULAR NEWS

  • ChatPandaGPT

    Insilico Medicine brings AI-powered “ChatPandaGPT” to its target discovery platform

    67 shares
    Share 27 Tweet 17
  • Northern and southern resident orcas hunt differently, which may help explain the decline of southern orcas

    44 shares
    Share 18 Tweet 11
  • Skipping breakfast may compromise the immune system

    43 shares
    Share 17 Tweet 11
  • Insular dwarfs and giants more likely to go extinct

    35 shares
    Share 14 Tweet 9

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Recent News

Seer researchers find undiscovered links between lung cancer and multiple distinct protein variants via deep, unbiased proteomics analysis

Ochsner Health named 2023 Gallup Exceptional Workplace Award Winner

New chip design to provide greatest precision in memory to date

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 48 other subscribers
  • Contact Us

Bioengineer.org © Copyright 2023 All Rights Reserved.

No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

Bioengineer.org © Copyright 2023 All Rights Reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In