In a study published in the journal Genes & Diseases, researchers from Army Medical University and Shenzhen University investigated the pivotal role of miR-18a-5p, a microRNA, in the development and progression of osteoblastic lesions resulting from prostate cancer (PCa) bone metastasis. They made a striking observation of significantly elevated miR-18a-5p expression in the bone microenvironment of PCa patients with bone metastases, indicating its potential involvement in the pathogenesis of the disease. To gain deeper insights into the impact of miR-18a-5p on osteoblastic lesions, the researchers conducted a series of comprehensive laboratory experiments. By inhibiting miR-18a-5p in both PCa cells and pre-osteoblasts, they successfully demonstrated a substantial reduction in osteoblast differentiation and activity. Particularly noteworthy was the administration of PCa cells with suppressed miR-18a-5p into a mouse model, which resulted in remarkable improvements in bone biomechanical properties and bone mineral mass, effectively highlighting the therapeutic potential of targeting this specific microRNA. Subsequent investigations unraveled the intricate molecular mechanism underlying the osteoblastic lesions induced by miR-18a-5p. The researchers discovered that this microRNA was transferred to osteoblasts via exosomes secreted by PCa cells. Within the osteoblasts, miR-18a-5p skillfully targeted the Hist1h2bc gene, leading to the up-regulation of Ctnnb1 in the Wnt/β-catenin signaling pathway, ultimately driving osteoblast differentiation and fostering the formation of osteoblastic lesions. The findings of this groundbreaking study hold immense promise for the development of novel and targeted therapeutic strategies for managing PCa bone metastasis and its associated osteoblastic complications. The researchers effectively employed antagomir-18a-5p, an inhibitor of miR-18a-5p, to ameliorate osteoblastic lesions in the mouse model, without adversely affecting osteoclast activity. Remarkably, antagomir-18a-5p treatment significantly improved bone biomechanical properties, bone mineral density, and alleviated sclerotic lesions, underscoring its potential efficacy as a promising treatment option for PCa-induced osteoblastic lesions in clinical settings. Given that prostate cancer bone metastasis represents a significant unmet medical need, especially concerning osteoblastic lesions, current treatments predominantly address osteolytic complications, leaving limited therapeutic options for osteoblastic manifestations. This groundbreaking research opens up new and exciting possibilities for targeted therapies that have the potential to significantly enhance the quality of life for PCa patients grappling with osteoblastic lesions.
Credit: Genes & Diseases
In a study published in the journal Genes & Diseases, researchers from Army Medical University and Shenzhen University investigated the pivotal role of miR-18a-5p, a microRNA, in the development and progression of osteoblastic lesions resulting from prostate cancer (PCa) bone metastasis. They made a striking observation of significantly elevated miR-18a-5p expression in the bone microenvironment of PCa patients with bone metastases, indicating its potential involvement in the pathogenesis of the disease. To gain deeper insights into the impact of miR-18a-5p on osteoblastic lesions, the researchers conducted a series of comprehensive laboratory experiments. By inhibiting miR-18a-5p in both PCa cells and pre-osteoblasts, they successfully demonstrated a substantial reduction in osteoblast differentiation and activity. Particularly noteworthy was the administration of PCa cells with suppressed miR-18a-5p into a mouse model, which resulted in remarkable improvements in bone biomechanical properties and bone mineral mass, effectively highlighting the therapeutic potential of targeting this specific microRNA. Subsequent investigations unraveled the intricate molecular mechanism underlying the osteoblastic lesions induced by miR-18a-5p. The researchers discovered that this microRNA was transferred to osteoblasts via exosomes secreted by PCa cells. Within the osteoblasts, miR-18a-5p skillfully targeted the Hist1h2bc gene, leading to the up-regulation of Ctnnb1 in the Wnt/β-catenin signaling pathway, ultimately driving osteoblast differentiation and fostering the formation of osteoblastic lesions. The findings of this groundbreaking study hold immense promise for the development of novel and targeted therapeutic strategies for managing PCa bone metastasis and its associated osteoblastic complications. The researchers effectively employed antagomir-18a-5p, an inhibitor of miR-18a-5p, to ameliorate osteoblastic lesions in the mouse model, without adversely affecting osteoclast activity. Remarkably, antagomir-18a-5p treatment significantly improved bone biomechanical properties, bone mineral density, and alleviated sclerotic lesions, underscoring its potential efficacy as a promising treatment option for PCa-induced osteoblastic lesions in clinical settings. Given that prostate cancer bone metastasis represents a significant unmet medical need, especially concerning osteoblastic lesions, current treatments predominantly address osteolytic complications, leaving limited therapeutic options for osteoblastic manifestations. This groundbreaking research opens up new and exciting possibilities for targeted therapies that have the potential to significantly enhance the quality of life for PCa patients grappling with osteoblastic lesions.
The study’s findings offer exciting prospects, but further research is needed to validate the safety and efficacy of this approach in humans. Nevertheless, it marks a significant step forward in combating the devastating impact of PCa bone metastasis and its osteoblastic lesions. With advancements in targeted therapies and drug delivery methods, this research could lead to improved treatments for PCa bone metastasis, bringing hope to patients, families, and healthcare professionals in the fight against prostate cancer and its complications.
###
References
DOI
10.1016/j.gendis.2022.06.007
Original Source URL
https://doi.org/10.1016/j.gendis.2022.06.007
Funding information
The National Natural Science Foundation of China (81930067), Key Project for Clinical Innovation of AMU (China) (CX2019LC107).
About Genes & Diseases
Genes & Diseases is a journal for molecular and translational medicine. The journal primarily focuses on publishing investigations on the molecular bases and experimental therapeutics of human diseases. Publication formats include full length research article, review article, short communication, correspondence, perspectives, commentary, views on news, and research watch.
Journal
Genes & Diseases
DOI
10.1016/j.gendis.2022.06.007
Subject of Research
Not applicable
Article Title
Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway
Article Publication Date
16-Jun-2023
COI Statement
The authors declare that they have no competing interests.
