Researchers led by Ramreddy Godela have developed and validated a reversed-phase high-performance liquid chromatography (RP-HPLC) technique capable of the simultaneous determination of rizatriptan benzoate and meloxicam. Published in BMC Chemistry, the analytical procedure incorporates an Analytical Quality by Design framework together with green analytical chemistry principles to deliver a stability-indicating assay.
The development strategy relied on a central composite design to systematically investigate and optimize critical method variables. Through this design approach, the researchers evaluated the influence of key operational parameters, focusing specifically on the flow rate of the mobile phase and the percentage of the organic modifier in the solvent mixture.
Chromatographic separation of the two active compounds was performed using a Shimpack C18 analytical column measuring 250 by 4.6 millimeters with a 5-micrometer particle size. The mobile phase consisted of a mixture of methanol and 0.1 percent formic acid prepared in an 80:20 volume-to-volume ratio, delivered at an operating flow rate of 1.0 milliliter per minute with spectrophotometric detection conducted at a wavelength of 296 nanometers.
Under these optimized chromatographic conditions, the assay achieved distinct separation between the analytes, yielding retention times of 1.89 minutes for rizatriptan benzoate and 7.97 minutes for meloxicam, corresponding to a chromatographic resolution of 23.3. Calibration evaluations demonstrated linear detector responses across concentrations ranging from 5 to 50 micrograms per milliliter, with correlation coefficients of 0.999 established for both rizatriptan benzoate and meloxicam.
The analytical protocol was comprehensively validated in accordance with International Council for Harmonisation guidelines, covering system suitability, linearity, precision, accuracy, robustness, specificity, sensitivity, and forced degradation testing. Recovery experiments yielded values ranging between 100.35 percent and 100.71 percent, while precision assessments produced percentage relative standard deviations between 0.62 percent and 0.98 percent, demonstrating high accuracy, precision, robustness, and specificity.
Sensitivity measurements identified limits of detection of 0.07 micrograms per milliliter for rizatriptan benzoate and 0.19 micrograms per milliliter for meloxicam. The corresponding limits of quantification were established at 0.20 micrograms per milliliter for rizatriptan benzoate and 0.50 micrograms per milliliter for meloxicam.
The environmental impact and practical applicability of the chromatographic procedure were appraised using multiple standardized metric frameworks. Greenness assessments produced an AGREE score of 0.70, a MoGAPI score of 80, and an Analytical Eco-Scale score of 77, confirming its eco-friendly design, while an evaluation under the Blue Applicability Grade Index yielded a score of 80, supporting the method as a simple, reliable, robust, and sustainable tool for routine pharmaceutical analysis.
Subject of Research: Simultaneous chromatographic determination of rizatriptan benzoate and meloxicam using green analytical quality by design principles
Article Title: AQbD Driven RP-HPLC method for estimating rizatriptan benzoate and meloxicam: integrating green and blue analytical approaches
Article References: Godela, R., Manumole, T. K., Kovvuri, L. S. S., & Bobba, N. (2026). AQbD Driven RP-HPLC method for estimating rizatriptan benzoate and meloxicam: integrating green and blue analytical approaches. BMC Chemistry, 20(1), Article 217. https://doi.org/10.1186/s13065-026-01893-z
Image Credits: AI Generated
DOI: 10.1186/s13065-026-01893-z
Keywords: Rizatriptan Benzoate, Meloxicam, RP-HPLC, Analytical Quality by Design, Central Composite Design, Green Analytical Chemistry, Blue Applicability Grade Index, AGREE Metric, MoGAPI, Analytical Eco-Scale, ICH Guidelines, Forced Degradation Studies, Shimpack C18 Column, Method Validation, Limit of Detection, Limit of Quantification
News Source: Bethany Barker. (November 14, 2026). Chemists Validate Sustainable Chromatography Method for Simultaneous Rizatriptan and Meloxicam Analysis. Scienmag.
