The little things matter: Chemists develop new sensor for microvolume pH detection

Measuring the pH of substances gives us vital clues about the world around us, such as identifying contaminated water or checking the toxicity of medical or cosmetic products.

Often, only small amounts of samples are available, but monitoring the variation in pH in these miniscule volumes matters. For example, identifying pH changes within tiny volumes of fluid from single cells can help in the detection of ovarian cancer.

However, the current methods for measuring pH are mainly for bulk solutions and are not sensitive enough or are too fragile to measure small volumes on a commercial scale.

In a recent study published in Microchimica Acta, scientists from Xi’an Jiaotong-Liverpool University, China, have developed a method that overcomes these problems.

Dr Qiuchen Dong, who led the study, says: “Our solution needed to be environmentally friendly, durable, and sensitive enough to accurately measure pH variation in just a few microlitres of samples.

A lack of options

Some commercially available methods used to test pH rely on the subjective decisions of the human eye. For example, using paper strips containing dyes that change colour depending on the pH of the substance relies on people comparing the colour against a scale. This results in significant variation in their answers. Some people may view the colour as pH 7.5, others at 8, for example. This method, therefore, is not sensitive to small pH changes, meaning it’s more like a rough guess. Some of the dyes used are also toxic to samples which will affect the pH recorded.

A more sensitive method of measuring pH uses extremely fragile glass electrodes, which are easily broken, so they are usually only used in a laboratory setting.

To solve these problems, Dr Dong and his postgraduate student Weiyu Xiao have used novel materials and methods to create a sensitive yet robust pH sensor.

A new solution

In Dr Dong and Xiao’s new pH sensor, fluid samples run through a series of tiny channels (microfluidic channels) and over three highly sensitive electrodes made out of light-responsive layered materials and metals.

“Our solution to the problem relies on developing microfluidic channels and electrodes using photolithography, a method often used in the semiconductor manufacturing industry.”

These microfluidic pH sensors can detect minor variations in the number of protons within a substance, which defines the pH. This allows the measurement of pH with excellent accuracy.

Future uses

The team currently has a patent pending for the pH sensor and is developing collaborations with industry developers who will integrate the technology into their lab equipment.

“The success of this study is down to the hard work of my current PhD student, Weiyu Xiao, who was a master’s student during this work. It is very impressive to see a student reach such a high level in such a short period. She is a great role model, and I hope other students will be inspired by how much she has achieved.

“The work is also thanks to my previous colleagues, Dr Abdennour Abbas at the University of Minnesota and Dr Yu Lei from the University of Connecticut, who helped me to formulate the ideas for this project and many others.”

The team believes that their new sensor will have extensive commercial applications, from aiding the detection of cancers and vector-borne viruses to identifying contamination in soil sprayed with pesticides.

About the researcher

Dr Qiuchen Dong’s expertise lies in the topics of electrochemical-based biosensors, chemical sensors, and gas sensors in the aspects of design, fabrication, and evaluation for biomedical and agricultural applications from the year 2013 to 2021.

Professor Yu Lei supervised Dr Dong during his graduate studies in Biomedical Engineering at the University of Connecticut from 2013-2019. Afterwards, Dr Dong moved to Minneapolis to work as a Postdoctoral Research Associate in Professor Abdennour Abbas’ lab in volatolomics in the Department of Bioproducts and Biosystems Engineering at the University of Minnesota, focusing on using Proton-transfer-reaction Mass Spectrometry (PTR-MS).

Dr Dong joined XJTLU in May 2021 and is now growing his lab to further explore various types of sensors, including microfluidics-based chemical sensors, nanomaterials-based electrochemical biosensors, and thin-film materials/nanoparticles/metal-organic-framework based gas sensors in the applications of biomedical and agricultural settings.