Credit: David Welch, Ph.D., Columbia University Vagelos College of Physicians and Surgeons
A specific wavelength of ultraviolet light, now delivered through light-diffusing optical fibers, is highly effective at killing drug-resistant bacteria in cell cultures, according to a new study led by David J. Brenner, PhD, a professor of radiation biophysics at Columbia University Vagelos College of Physicians and Surgeons. The technology is designed to prevent infections around skin-penetrating medical devices, such as catheters or mechanical heart pump drivelines.
Why it Matters
Infections from skin-penetrating medical devices, including catheters and drivelines for left ventricular assist devices (LVADs), are a major health threat. For example, an estimated 14 to 28 percent of patients with an LVAD develops a driveline skin infection, leading to complications that limit their use as a long-term therapy for heart failure patients. The most serious of these infections are caused by the drug-resistant bacteria MRSA (methicillin-resistant Staphylococcus aureus).
Background
In a previous mouse study, Brenner and his Columbia team demonstrated that a narrow spectrum of far-UVC light, with a wavelength of 207 to 224 nanometers (nm), can kill MRSA bacteria without damaging human skin. Conventional germicidal UV light, with a wavelength of 254 nm, is also effective at killing bacteria, but it can't be used in health care settings around people because it can harm the skin and eyes. Far-UVC light is safe for people because it can't penetrate the outer layer of dead skin or the tear layer of the eye, but it's deadly for bacteria, which are much smaller and easier to penetrate.
What's New
The current study was designed to test whether far-UVC light that is transmitted along a thin fiber could be used to disinfect complex tissue shapes, such as the area where a catheter or a driveline enters the skin. The Columbia team developed a new way to deliver the light, using a laser to send 224 nm far-UVC light through a thin flexible optical fiber. In this study, the fibers were laid directly over tissue cultures containing MRSA bacteria, which were efficiently killed by the far-UVC light diffusing out of the fibers.
What it Means
"Our study suggests that far-UVC light, delivered by optical fibers that can be incorporated into skin-penetrating devices, could be used to prevent catheter-based and driveline infections," said Brenner. "This application would be used for catheters or drivelines that have to be kept in place for long periods of time, and it's hard to keep the area where they penetrate the skin sterile. Incorporating these thin far-UVC-emitting fibers into the catheter or driveline may be the solution."
What's Next
Studies to determine if the technology can prevent infections around skin-penetrating lines in animal models are currently underway.
Caveats
The study was performed on bacteria in laboratory tissue cultures, not on living animals or human patients. In addition, the technology to make the equipment easily portable and affordable is under development.
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More Details
David J. Brenner, PhD, is the Higgins Professor of Radiation Biophysics and of environmental health sciences and director of the Center for Radiological Research at Columbia University Irving Medical Center.
The study, "Effect of far ultraviolet light emitted from an optical diffuser on methicillin-resistant Staphylococcus aureus in vitro," was published August 10 in PLOS ONE.
Other authors (all Columbia University Vagelos College of Physicians and Surgeons): David Welch, Manuela Buonanno, Igor Shuryak, Gerhard Randers-Pehrson, and Henry M. Spotnitz.
The work was supported by the Shostack Foundation and the Columbia-Coulter Translational Research Partnership.
Columbia University Irving Medical Center provides international leadership in basic, preclinical, and clinical research; medical and health sciences education; and patient care. The medical center trains future leaders and includes the dedicated work of many physicians, scientists, public health professionals, dentists, and nurses at the Vagelos College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing, the biomedical departments of the Graduate School of Arts and Sciences, and allied research centers and institutions. Columbia University Irving Medical Center is home to the largest medical research enterprise in New York City and State and one of the largest faculty medical practices in the Northeast. For more information, visit cumc.columbia.edu or columbiadoctors.org.
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Related Journal Article
http://dx.doi.org/10.1371/journal.pone.0202275
