A groundbreaking study emerging from the laboratories of Johns Hopkins Medicine elucidates a novel neuroprotective function of the enzyme biliverdin reductase A (BVRA). While traditionally recognized for its enzymatic role in the conversion of biliverdin to bilirubin—a yellow pigment with known antioxidant properties—this new research discloses that BVRA exerts a critical protective influence against oxidative stress in neurons independent of bilirubin production. This discovery opens new avenues for therapeutic strategies aimed at neurodegenerative diseases marked by oxidative damage, such as Alzheimer’s disease.
Oxidative stress is an imbalance between reactive oxygen species and the antioxidant defenses of cells, which progressively impairs cellular function and viability, particularly in the brain. BVRA has now been identified as a potent modulator of the nuclear factor erythroid 2-related factor 2 (NRF2), a master regulator of antioxidant response elements in the genome. NRF2 controls the expression of a suite of genes involved in detoxification, antioxidant generation, and overall cellular resilience. The intersection between BVRA and NRF2 delineates a crucial juncture in neuroprotection, independent of the classic bilirubin pathway.
This insight arose from meticulous studies involving genetically engineered murine models. Mice were created with deletions in genes encoding both BVRA and NRF2, resulting in non-viable progeny, a compelling indication of the interdependence of these proteins for survival. Subsequent experiments targeting BVRA alone revealed a disruption in NRF2’s normal function, manifested as diminished expression of NRF2 target genes critical for antioxidant defense mechanisms. These observations underscore a functional synergy where BVRA stabilizes or facilitates NRF2 activity at a molecular level.
Cellular investigations further substantiated these findings. In vitro models demonstrated a physical interaction between BVRA and NRF2 proteins, suggesting a direct binding relationship. This binding was shown to regulate the transcription of downstream genes pivotal not only for oxidative defense but also for processes such as oxygen transport, immune signaling, and mitochondrial electron transport chain efficiency—highlighting BVRA as a central integrator of multiple cellular pathways essential for maintaining neuronal health.
Remarkably, the neuroprotective actions of BVRA persisted even when the enzyme’s capacity to synthesize bilirubin was experimentally abolished. Mutant forms of BVRA incapable of bilirubin production maintained their regulatory effect on NRF2 and conferred neuronal protection, decisively separating BVRA’s antioxidant regulatory function from bilirubin biosynthesis. This non-canonical role of BVRA redefines our molecular understanding of neuronal defense strategies.
These findings bear profound implications for neurodegenerative disease research and drug development. Targeting the BVRA-NRF2 axis could constitute a novel therapeutic approach to slow or mitigate neurodegeneration in diseases where oxidative stress is a pathological hallmark, including Alzheimer’s disease. Pharmacological agents designed to enhance BVRA’s interaction with NRF2, or mimic its effects, might bolster intrinsic neuronal resistance to oxidative injury.
The study not only advances molecular neuroscience but also highlights the indispensable value of long-term, mechanistic biomedical research. The multidisciplinary collaboration spanning neuroscience, biochemistry, genomics, and clinical medicine was crucial for unraveling this complex biological interplay, illustrating how comprehensive expertise can spearhead discoveries with far-reaching clinical potential.
Future research directions aim to dissect how the BVRA-NRF2 relationship becomes dysregulated in pathological states. In particular, exploring this interaction in Alzheimer’s disease models will clarify whether modulating this pathway can attenuate disease progression or cognitive decline. Such investigations could pave the way for precision medicine approaches tailored to enhancing endogenous antioxidant defenses in vulnerable neuronal populations.
The scientific team’s effort represents years of dedicated inquiry backed by substantial funding from prestigious institutions including the National Institutes of Health, American Heart Association, and several foundations committed to advancing brain health and cognitive impairment research. These sustained investments underscore the critical importance of supporting foundational science to unlock therapeutic innovations.
Notably, this work corroborates and expands upon earlier findings that identified bilirubin as an antioxidant in the brain, as well as studies revealing the pigment’s protective effects against severe malaria pathology. By decoupling BVRA’s enzymatic function from its regulatory influence on NRF2, this research redefines the paradigm of antioxidant biology in neural tissues with potential translational impact.
In conclusion, BVRA emerges not merely as an enzymatic catalyst but as a multifaceted molecular integrator that orchestrates critical cellular defense networks. This pivotal role emphasizes the enzyme’s potential as a therapeutic target aimed at enhancing neuronal resilience in the face of oxidative stress and neurodegenerative insults, thus illuminating a promising pathway toward combating debilitating brain disorders.
Subject of Research: Neuroprotection, Oxidative stress, Biliverdin reductase A, NRF2 regulation, Neurodegenerative diseases
Article Title: Johns Hopkins Scientists Reveal Biliverdin Reductase A as a Novel Neuroprotective Modulator of NRF2 Independent of Bilirubin Synthesis
News Publication Date: September 30, 2025
Web References: https://www.pnas.org/doi/10.1073/pnas.2513120122
References: Previous NIH-funded studies published in Cell Chemical Biology and Science regarding bilirubin’s antioxidant role and protective effects against malaria
Keywords: Redox processes, Protein functions, Oxidative stress, BVRA, NRF2, Neurodegeneration, Antioxidant defense, Alzheimer’s disease, Mitochondrial function, Neuroprotection
Tags: antioxidant response in brain healthbiliverdin to bilirubin conversionbrain health and antioxidant defensescellular function and oxidative damagedual role of biliverdin reductase Agenetic engineering in neuroscience researchimplications for Alzheimer’s disease treatmentneuroprotection and oxidative stress mechanismsneuroprotective functions of BVRANRF2 regulation in cellular resilienceoxidative stress in neuronstherapeutic strategies for neurodegenerative diseases
