The LOH-Theory is based on the following original discoveries: highly-concentrated semi-liquid water systems saturated with functional organic substances have, at around 290 K, the so-called gas-hydrate honeycomb structure consisting of large (0.69 nm) and small (0.48 nm) cavities similar to the structure of underground methane deposits. DNA (RNA) functional groups are in three-dimensional size accordance with the gas-hydrate structural cavities; CH4-hydrate with its S-containing admixtures and NO3? — and PO43? — ions represent the unique triad of widely distributed minerals allowing exothermal and thermodynamically feasible syntheses of nucleic acids, amino-acids, proteins, lipids, monosaccharides, polysaccharides, tissues, and cells and are supported by analyses of available literature, including paleontological data on the periods of appearances, developments and prosperities, and degradations and extinctions of Earth's flora and fauna.
According to the LOH-Theory, nitrogen bases (N-bases) and ribose molecules were synthesized within the large and small CH4-hydrate cavities, respectively, with nucleoside formation as a result of NO3?- ion diffusion into the hydrate structure. The phosphate bridges arose within small cavities as a result of PO43?- ion diffusion into the structure, resulting in formation of DNA- and RNA-like molecules with different sequences of N-bases. Due to reaction water and heat evolution, the structure liquated, and subsequent reactions, including elementary symbiotic processes, proceeded within the semi-liquid super-protoplasm. Cells, amino-acids, first proteins, and primitive organisms arose in this medium, owing to preservation of the hydrate matrix and to diffusion of the same triad of minerals from the outside. A multitude of DNA molecules with different sequences of N-bases originated in each CH4-hydrate localization. During the historic periods favorable for living-matter origination, different DNAs formed within many localizations.
The LOH-Theory proposes experimental procedure for living-matter synthesis from minerals.
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Reference: Kadyshevich, EA.; (2017). Life Origination Hydrate Theory (LOH-Theory): Thermodynamic and Kinetic Factors in the Natural Mechanisms of Amino-Acids Origination, DOI: 10.2174/1570193X13666151125231015
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