• HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
Friday, October 3, 2025
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News Biology

Better gene knockout in aneuploid cell lines

Bioengineer by Bioengineer
May 23, 2019
in Biology
Reading Time: 3 mins read
0
IMAGE
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram

IMAGE

Credit: ©2012, Mary Ann Liebert, Inc., publishers

New Rochelle, NY, May 15, 2019-CRISPR/Cas9 technology enables convenient and effective genome editing in diploid cell lines based on the isolation and expansion of edited single-cell clones. However, this approach is ineffective for aneuploid cell lines, and a group has now reported an improved method for genome editing based on multiple rounds of modification. The article is published in Tissue Engineering, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. Click here to read the article for free on the Tissue Engineering website until June 15, 2019.

Maxim N. Karagyaur, MD, PhD, Lomonosov Moscow State University, Russia, and a team of colleagues present their research in an article titled “Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines”. The authors treated four different human and mouse aneuploid cell lines in pooled populations and knocked out a combined six different genes. Their newly developed protocol includes repeated rounds of genome editing and GFP-positive cell sorting. Both on-target and off-target editing was assessed by sequencing predicted sites, and the results showed that additional rounds did not increase the rate of off-target cleavage despite enhancing gene knockout efficacy.

“Dr. Karagyaur and colleagues from Russia describe a critical development in CRISPR/Cas9 technology to create functional cell models from aneuploid cell cultures,” says Tissue Engineering Co-Editor-in-Chief John P. Fisher, PhD, Fischell Family Distinguished Professor & Department Chair, and Director of the NIH Center for Engineering Complex Tissues at the University of Maryland. “Utilizing an innovative approach of repetitive cycles of CRISPR-/Cas9, the group showed that target protein expression could be suppressed, and therefore bypass clonal effects which are often observed in these complex systems.”

###

About the Journal

Tissue Engineering is an authoritative peer-reviewed journal published monthly online and in print in three parts: Part A, the flagship journal published 24 times per year; Part B: Reviews, published bimonthly, and Part C: Methods, published 12 times per year. Led by Co-Editors-in-Chief Antonios G. Mikos, PhD, Louis Calder Professor at Rice University, Houston, TX, and John P. Fisher, PhD, Fischell Family Distinguished Professor & Department Chair, and Director of the NIH Center for Engineering Complex Tissues at the University of Maryland, the Journal brings together scientific and medical experts in the fields of biomedical engineering, material science, molecular and cellular biology, and genetic engineering. Leadership of Tissue Engineering Parts B (Reviews) and Part C (Methods) is provided by Katja Schenke-Layland, PhD, Eberhard Karls University, Tübingen, Heungsoo Shin, PhD, Hanyang University; and John A. Jansen, DDS, PhD, Radboud University, and Xiumei Wang, PhD, Tsinghua University respectively. Tissue Engineering is the official journal of the Tissue Engineering & Regenerative Medicine International Society (TERMIS). Complete tables of content and a sample issue may be viewed on the Tissue Engineering website.

About the Publisher

Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative peer-reviewed journals in many promising areas of science and biomedical research, including Stem Cells and Development, Human Gene Therapy, and Advances in Wound Care. Its biotechnology trade magazine, GEN (Genetic Engineering & Biotechnology News), was the first in its field and is today the industry’s most widely read publication worldwide. A complete list of the firm’s 80 journals, books, and newsmagazines is available on the Mary Ann Liebert, Inc., publishers website.

Media Contact
Kathryn Ryan
[email protected]

Tags: BiologyCell BiologyGene Therapy
Share12Tweet8Share2ShareShareShare2

Related Posts

Encapsulated Pseudomonas Controls Pistachio Gummosis Effectively

Encapsulated Pseudomonas Controls Pistachio Gummosis Effectively

October 3, 2025
Scientists Uncover New Intracellular Trafficking Pathway in Plant Cells

Scientists Uncover New Intracellular Trafficking Pathway in Plant Cells

October 3, 2025

Microscopic Sugars in the Brain Alter Emotional Pathways, Driving Depression

October 3, 2025

Plant Mobile Domain Proteins Resist Polycomb Gene Silencing

October 3, 2025
Please login to join discussion

POPULAR NEWS

  • New Study Reveals the Science Behind Exercise and Weight Loss

    New Study Reveals the Science Behind Exercise and Weight Loss

    93 shares
    Share 37 Tweet 23
  • New Study Indicates Children’s Risk of Long COVID Could Double Following a Second Infection – The Lancet Infectious Diseases

    88 shares
    Share 35 Tweet 22
  • Physicists Develop Visible Time Crystal for the First Time

    75 shares
    Share 30 Tweet 19
  • New Insights Suggest ALS May Be an Autoimmune Disease

    67 shares
    Share 27 Tweet 17

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Recent News

Encapsulated Pseudomonas Controls Pistachio Gummosis Effectively

Illuminating the Future: Transforming Streetlamps into Electric Vehicle Chargers

Transforming Palm Waste into High-Performance CO₂ Absorbers: Malaysian Scientists Innovate with Agricultural Byproducts

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 62 other subscribers
  • Contact Us

Bioengineer.org © Copyright 2023 All Rights Reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In
No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

Bioengineer.org © Copyright 2023 All Rights Reserved.