At the heart of the TME’s complexity lies the dual nature of ferroptosis: it is both a weapon against malignant cells and a modulator of immune function. Cytotoxic CD8+ T cells are now known to potentiate antitumor activity by releasing interferon-gamma (IFN-γ), which downregulates the system xc– cystine/glutamate antiporter in cancer cells, thereby depleting glutathione (GSH)—a key antioxidant. This depletion sensitizes tumor cells to ferroptosis, revealing a novel immunological mechanism of tumor suppression. Moreover, IFN-γ influences the phenotype of tumor-associated macrophages (TAMs), driving their transformation toward the pro-inflammatory M1 subtype that supports tumor eradication and impedes cancer progression.
Conversely, immune cells themselves are not impervious to ferroptosis within the TME. CD8+ and CD4+ T cells exhibit lipid peroxidation under conditions of impaired glutathione peroxidase 4 (GPX4) activity or exposure to ferroptosis inducers like RSL3, resulting in compromised immune function. Strikingly, overexpression of protective proteins such as GPX4 and ferroptosis suppressor protein 1 (FSP1) shields these lymphocytes from ferroptotic death. These insights underscore the delicate balance wherein immune cells navigate oxidative stress—not merely as bystanders but as active participants whose survival directly impacts antitumor immunity.
Regulatory T cells (Tregs), notorious for suppressing immune responses, also intertwine with ferroptosis pathways. In the absence of GPX4, Tregs demonstrate heightened ferroptotic sensitivity, leading to the secretion of pro-inflammatory cytokines such as IL-1β, which paradoxically facilitates the expansion of tumor-promoting T helper 17 (Th17) cells. This phenomenon illustrates how ferroptosis modulation within Tregs could recalibrate the immunosuppressive landscape of the TME; however, the therapeutic challenge remains to selectively target tumor-infiltrating Tregs without unleashing systemic autoimmunity.
B cells, especially the marginal zone and B1 subsets, have recently been implicated in ferroptotic regulation within tumors. These cells’ reliance on fatty acid uptake through scavenger receptors like CD36 predisposes them to lipid peroxide accumulation and ferroptosis when GPX4 activity wanes. The metabolic reprogramming intrinsic to their survival and function adds a further layer of complexity, suggesting that ferroptosis not only shapes lymphocyte fate but also influences humoral responses in cancer contexts.
Dendritic cells (DCs), vital for antigen presentation and T cell activation, are vulnerable to ferroptotic damage wrought by oxidative stress and lipid peroxidation by-products. This accumulation triggers endoplasmic reticulum stress and engages transcriptional programs such as the X-box binding protein 1 (XBP1) pathway, undermining DCs’ immunostimulatory capacity. Intriguingly, ferroptosis in DCs can be mitigated by blocking peroxisome proliferator-activated receptor gamma (PPARγ), opening avenues to preserve their tumor-fighting potential in the oxidative TME milieu.
Natural killer (NK) cells, crucial innate effectors, face ferroptotic threats primarily through lipid peroxidation triggered by tumor metabolites like L-Kynurenine. This lipid oxidative stress impairs NK cell glycolysis—a metabolic pathway essential for their cytotoxic function. Protective factors such as GPX4 overexpression and nuclear factor erythroid 2–related factor 2 (NRF2) activation can rescue NK cells from ferroptosis and restore their antitumor efficacy. These mechanistic insights provide a foundation for enhancing NK cell resilience in hostile tumor niches.
Myeloid-derived suppressor cells (MDSCs), particularly polymorphonuclear subsets, undergo spontaneous ferroptosis in the TME due to heightened oxidative stress and GPX4 downregulation. While ferroptosis reduces MDSC numbers, the release of immunosuppressive lipid mediators like prostaglandin E2 (PGE2) following cell death paradoxically hinders antitumor T cell activity and supports TAM-mediated immune evasion. Thus, ferroptosis in MDSCs presents a double-edged sword, demanding nuanced therapeutic interventions that consider downstream immunomodulatory effects.
Collectively, the dynamic interactions between ferroptosis and the multifarious cell types within the TME underscore an intricate regulatory network with profound implications for cancer biology. Therapeutic approaches leveraging ferroptosis must, therefore, consider impacts not only on tumor cells but also on immune and stromal compartments that critically modulate antitumor immunity. Targeted induction of ferroptosis in tumor cells combined with preservation or restoration of immune cell function holds promise for next-generation cancer therapies.
The emerging paradigm situates ferroptosis as a nexus connecting metabolic reprogramming, oxidative stress, and immune regulation. Beyond its cytotoxic role, ferroptosis shapes the immunological milieu, influencing antigen presentation, immune cell polarization, and cytokine milieu, thereby dictating either tumor suppression or progression. Enhancing our mechanistic understanding will facilitate the design of precision interventions that harness ferroptosis within the immune contexture of tumors.
Future research priorities include developing selective ferroptosis modulators capable of discriminating between pro-tumorigenic and anti-tumorigenic cell populations, optimizing delivery systems such as ferroptosis-inducing nanoparticles, and integrating ferroptosis-targeted therapies with immune checkpoint blockade. Additionally, deeper insights into metabolic dependencies that predispose immune subsets to ferroptotic death will enable strategies to bolster immune resilience amidst TME oxidative challenges.
Subject of Research:
Ferroptosis and its complex role within the tumor microenvironment, focusing on interactions between immune cells and cancer cells in esophageal cancer.
Article Title:
Exploring the role of ferroptosis in esophageal cancer: mechanisms and therapeutic implications.
Article References:
Zhao, D., Li, W., Han, Z. et al. Exploring the role of ferroptosis in esophageal cancer: mechanisms and therapeutic implications. Cell Death Discov. 11, 405 (2025). https://doi.org/10.1038/s41420-025-02696-2
Image Credits:
AI Generated
DOI:
https://doi.org/10.1038/s41420-025-02696-2
Tags: cancer therapy strategiesCD8+ T cell antitumor activityesophageal cancer treatment advancementsferroptosis in esophageal cancerglutathione depletion and ferroptosisimmune cell interactions in cancerimmunological mechanisms of tumor suppressioniron-dependent cell death mechanismslipid peroxidation in tumorspro-inflammatory immune responses in cancertumor microenvironment dynamicstumor-associated macrophage polarization
