A joint team of geneticists from the University of Geneva (UNIGE) and the Swiss Federal Institute of Technology in Lausanne (EPFL), Switzerland, demonstrated that the emergence of mammary glands in placental mammals and marsupials results from recycling certain 'architect' genes. The latter, known as Hox genes, are responsible for coordinating the formation of the organs and limbs during the embryonic stage. Such genes are controlled by complex regulatory networks. In the course of evolution, parts of these networks were reused to produce different functions. Architect genes were thus requisitioned to form the mammary bud and, later, for gestation. This team's work has been published in the journal PNAS.
An ubiquitous regulatory module
The group led by Denis Duboule, a geneticist at UNIGE and EPFL, is interested in the mechanisms involved in the appearance of mammary glands. As the professor notes, 'We observed that certain architect genes are at work during the development of the mammary bud in mice, and we wanted to understand how and why'.
The research team had previously identified a section of DNA adjacent to the HoxD gene cluster, which formed a particular 3D structure in order to interact with and activate certain Hox genes. 'We established that this particular DNA sequence adopts the same three-dimensional structure in tissues as diverse as those destined to become arms, intestines or mammary glands', says Leonardo Beccari, a member of the Geneva team and the project leader.
This does not concern the platypus
The three-dimensional conformation of this DNA is identical to that of other organs but we still had to understand what characterised the regulatory mechanism for the Hox genes in the mammary bud, which appeared later in the evolutionary cycle. 'We discovered the existence of a short DNA sequence capable of activating a specific Hox gene, and which is present only in placental mammals and marsupials', explains Ruben Schep, the first author of the article. Indeed, mammals that lay eggs, such as the platypus, do not have this sequence.
This short DNA sequence, called MBRE, was only able to carry out its function because it appeared in a region of this three-dimensional DNA where there was already a contact with genes from the HoxD group. Control of these genes' expression in the mammary bud has thus evolved thanks to the hijacking of this pre-existing regulatory module, thereby explaining the later arrival of such a structure, and thus of the placental mammals and the marsupials .
###
Media Contact
Denis Duboule
[email protected]
41-223-796-771
@UNIGEnews
http://www.unige.ch
############
Story Source: Materials provided by Scienmag
