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		<title>Ribosomal RNA Passes Its Test as a Steady Reference in High-Throughput Plasma qPCR</title>
		<link>https://bioengineer.org/ribosomal-rna-passes-its-test-as-a-steady-reference-in-high-throughput-plasma-qpcr/</link>
		
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		<pubDate>Mon, 05 Oct 2026 06:21:22 +0000</pubDate>
				<category><![CDATA[Biology]]></category>
		<category><![CDATA[18S rRNA]]></category>
		<category><![CDATA[biomarkers]]></category>
		<category><![CDATA[Bland-Altman analysis]]></category>
		<category><![CDATA[endogenous control]]></category>
		<category><![CDATA[FIELD trial]]></category>
		<category><![CDATA[gene expression normalisation]]></category>
		<category><![CDATA[OpenArray]]></category>
		<category><![CDATA[plasma RNA]]></category>
		<category><![CDATA[pre-amplification]]></category>
		<category><![CDATA[qPCR]]></category>
		<category><![CDATA[reference gene]]></category>
		<category><![CDATA[Technical validation]]></category>
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					<description><![CDATA[A large analysis of FIELD trial plasma samples shows that 18 S rRNA is a technically stable qPCR control that does not bias co-amplified targets during multiplex pre-amplification, though archived and new primer pools differ enough to warrant caution in longitudinal studies.]]></description>
		
		
		
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