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	<title>gene normalization &#8211; BIOENGINEER.ORG</title>
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		<title>Hamster blood study reveals hidden flaw in gene expression analysis during infection and vaccination</title>
		<link>https://bioengineer.org/hamster-blood-study-reveals-hidden-flaw-in-gene-expression-analysis-during-infection-and-vaccination/</link>
		
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		<pubDate>Mon, 26 Oct 2026 01:27:24 +0000</pubDate>
				<category><![CDATA[Biology]]></category>
		<category><![CDATA[ACTB]]></category>
		<category><![CDATA[blood transcriptomics]]></category>
		<category><![CDATA[gene normalization]]></category>
		<category><![CDATA[golden Syrian hamster]]></category>
		<category><![CDATA[Leptospira interrogans]]></category>
		<category><![CDATA[leptospirosis]]></category>
		<category><![CDATA[PPIA]]></category>
		<category><![CDATA[reference genes]]></category>
		<category><![CDATA[RefSeeker]]></category>
		<category><![CDATA[RT-qPCR]]></category>
		<category><![CDATA[TNF-alpha]]></category>
		<category><![CDATA[vaccination]]></category>
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					<description><![CDATA[Researchers identified ppia as the most stable reference gene for RT-qPCR normalization in golden Syrian hamster blood, showing that the commonly used actb gene can overestimate inflammatory gene expression by roughly tenfold.]]></description>
		
		
		
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