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	<title>cold shock pre-treatment &#8211; BIOENGINEER.ORG</title>
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		<title>Cold Shock and a Growth Regulator Crack the Haploid Bottleneck in African Marigold Breeding</title>
		<link>https://bioengineer.org/cold-shock-and-a-growth-regulator-crack-the-haploid-bottleneck-in-african-marigold-breeding/</link>
		
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		<pubDate>Wed, 07 Oct 2026 11:07:23 +0000</pubDate>
				<category><![CDATA[Agriculture]]></category>
		<category><![CDATA[African marigold]]></category>
		<category><![CDATA[cold shock pre-treatment]]></category>
		<category><![CDATA[doubled haploid]]></category>
		<category><![CDATA[F1 hybrid breeding]]></category>
		<category><![CDATA[gynogenesis]]></category>
		<category><![CDATA[haploid]]></category>
		<category><![CDATA[ICAR-IARI]]></category>
		<category><![CDATA[ovary culture]]></category>
		<category><![CDATA[plant tissue culture]]></category>
		<category><![CDATA[ploidy analysis]]></category>
		<category><![CDATA[Tagetes erecta]]></category>
		<category><![CDATA[thidiazuron]]></category>
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					<description><![CDATA[Researchers at ICAR-IARI have for the first time regenerated haploid African marigold plants directly from unfertilized ovaries using cold shock and optimized thidiazuron treatment, opening a faster route to homozygous parental lines for hybrid breeding.]]></description>
		
		
		
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