Variation in brain wiring contributes to non-heritable behavioral individuality. How and when these individualized wiring patterns emerge and stabilize during development remains unexplored. In this study, we investigated the axon targeting dynamics of Drosophila visual projecting neurons called DCNs/LC14s, using four-dimensional live-imaging, mathematical modeling and experimental validation. We found that alternative axon targeting choices are driven by a sequence of two independent genetically encoded stochastic processes. Early Notch lateral inhibition segregates DCNs into NotchON proximally targeting axons and NotchOFF axons that adopt a bi-potential transitory state. Subsequently, probabilistic accumulation of stable microtubules in a fraction of NotchOFF axons leads to distal target innervation, whereas the rest retract to adopt a NotchON target choice. The sequential wiring decisions result in the stochastic selection of different numbers of distally targeting axons in each individual. In summary, this work provides a conceptual and mechanistic framework for the emergence of individually variable, yet robust, circuit diagrams during development.
Andriatsilavo, M., Barata, C., Reifenstein, E. et al. Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
Nat Neurosci (2025).
bu içeriği en az 2500 kelime olacak şekilde ve alt başlıklar ve madde içermiyecek şekilde ünlü bir science magazine için İngilizce olarak yeniden yaz. Teknik açıklamalar içersin ve viral olacak şekilde İngilizce yaz. Haber dışında başka bir şey içermesin. Haber içerisinde en az 14 paragraf ve her bir paragrafta da en az 80 kelime olsun. Cevapta sadece haber olsun. Ayrıca haberi yazdıktan sonra içerikten yararlanarak aşağıdaki başlıkların bilgisi var ise haberin altında doldur. Eğer bilgi yoksa ilgili kısmı yazma.:
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Andriatsilavo, M., Barata, C., Reifenstein, E. et al. Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
Nat Neurosci (2025). https://doi.org/10.1038/s41593-025-01937-y
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Tags: axon targeting dynamicsbehavioral individuality in fruit fliesbrain wiring individualitydevelopmental neuroscience researchdifferential axon targeting mechanismsDrosophila melanogaster developmentfour-dimensional live-imaging techniquesmicrotubule accumulation in axonsNotch signaling in neuronsprobabilistic axon innervationstochastic processes in neurosciencevisual projecting neurons
