• HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
Wednesday, May 31, 2023
BIOENGINEER.ORG
No Result
View All Result
  • Login
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
  • HOME
  • NEWS
  • EXPLORE
    • CAREER
      • Companies
      • Jobs
        • Lecturer
        • PhD Studentship
        • Postdoc
        • Research Assistant
    • EVENTS
    • iGEM
      • News
      • Team
    • PHOTOS
    • VIDEO
    • WIKI
  • BLOG
  • COMMUNITY
    • FACEBOOK
    • INSTAGRAM
    • TWITTER
  • CONTACT US
No Result
View All Result
Bioengineer.org
No Result
View All Result
Home NEWS Science News

Bioprinting for bone repair improved with genes

Bioengineer by Bioengineer
April 12, 2022
in Science News
Reading Time: 3 mins read
0
Share on FacebookShare on TwitterShare on LinkedinShare on RedditShare on Telegram

Given enough time and energy, the body will heal, but when doctors or engineers intervene, the processes do not always proceed as planned because chemicals that control and facilitate the healing process are missing. Now, an international team of engineers is bioprinting bone along with two growth factor encoding genes that help incorporate the cells and heal defects in the skulls of rats.

bbioprinting

Credit: Dong Heo, Kyung Hee University; Ozbolat Lab, Penn State. / Penn State

Given enough time and energy, the body will heal, but when doctors or engineers intervene, the processes do not always proceed as planned because chemicals that control and facilitate the healing process are missing. Now, an international team of engineers is bioprinting bone along with two growth factor encoding genes that help incorporate the cells and heal defects in the skulls of rats.

“Growth factors are essential for cell growth,” said Ibrahim T. Ozbolat, associate professor of engineering science and mechanics. “We use two different genes encoding two different growth factors. These growth factors help stem cells to migrate into the defect area and then help the progenitor cells to convert into bone.”

The researchers used gene encoding PDGF-B, platelet derived-growth factor, which encourages cells to multiply and to migrate, and gene encoding BMP-2, bone morphogenetic protein, which improves bone regeneration. They delivered both genes using bioprinting.

“We used a controlled co-delivery release of plasmids from a gene-activated matrix to promote bone repair,” the researchers stated in the journal Biomaterials.

Ozbolat and his team embedded the DNA for the protein in plasmids — ringlike loops of DNA that can transport genetic information. Once the DNA enters the progenitor cell, it begins to produce the appropriate proteins to enhance bone growth.

The two genes were printed during surgery onto a hole in the skull of a rat using a device very similar to an ink-jet printer. The mixture was created to release a burst of PDGF-B encoding gene in 10 days and a continuing release of BMP-2 encoding gene for five weeks. 

The rats that received bioprinted genes with controlled release of BMP-2 encoding gene saw about 40% bone tissue creation and 90% bone coverage in six weeks compared to 10% new bone tissue and 25% bone coverage for rats with the same defect, but no treatment.

“This method is better than simply dumping the growth factors,” said Ozbolat. “If we do that, the amounts of proteins are finite, but if we use gene therapy, the cells continue to produce the necessary growth factors.”

Working with Ozbolat from Penn State were Kazim K. Moncal, graduate student in engineering science and mechanics; Gregory S. Lewis, assistant professor and Hwabok Wee, postdoctoral fellow both in orthopedics and rehabilitation; Kevin P. Godzik, undergraduate in biomedical engineering: and Elias Rizk, associate professor of neurosurgery.

Others contributing to the research include R. Seda Tigli Aydin, former Penn State postdoctoral fellow now at Bulen Ecevit University, Turkey; Dong N. Heo, former Penn State postdoctoral fellow now at the Kyung Hee University, South Korea; and Timothy M. Acri, former graduate researcher, and Aliasger K. Salem, Lyle and Sharon Bighley Endowed Chair & Professor in Pharmaceutical Sciences, University of Iowa.

The International Team for Implantology, the National Institutes of Health, the National Science Foundation, the Osteology Foundation and the Scientific and Technological Research Council of Turkey supported this work.



Journal

Biomaterials

DOI

10.1016/j.biomaterials.2021.121333

Article Title

Controlled Co-delivery of pPDGF-B and pBMP-2 from intraoperatively bioprinted bone constructs improves the repair of calvarial defects in rats

Article Publication Date

1-Feb-2022

Share12Tweet8Share2ShareShareShare2

Related Posts

Anomalodonta and vanuxemia

The clams that fell behind, and what they can tell us about evolution and extinction

May 31, 2023
Figure 1. Design of the proposed microfluidic device for observing dynamic flows in the intestine.

Shedding light on the complex flow dynamics within the small intestine

May 31, 2023

Genetic change increased bird flu severity during U.S. spread

May 30, 2023

Matthew Bailes, Duncan Lorimer and Maura McLaughlin receive the 2023 Shaw Prize in Astronomy

May 30, 2023

POPULAR NEWS

  • plants

    Plants remove cancer causing toxins from air

    39 shares
    Share 16 Tweet 10
  • Element creation in the lab deepens understanding of surface explosions on neutron stars

    36 shares
    Share 14 Tweet 9
  • Groundbreaking study uncovers first evidence of long-term directionality in the origination of human mutation, fundamentally challenging Neo-Darwinism

    115 shares
    Share 46 Tweet 29
  • How life and geology worked together to forge Earth’s nutrient rich crust

    35 shares
    Share 14 Tweet 9

About

We bring you the latest biotechnology news from best research centers and universities around the world. Check our website.

Follow us

Recent News

The clams that fell behind, and what they can tell us about evolution and extinction

Shedding light on the complex flow dynamics within the small intestine

Genetic change increased bird flu severity during U.S. spread

Subscribe to Blog via Email

Enter your email address to subscribe to this blog and receive notifications of new posts by email.

Join 50 other subscribers
  • Contact Us

Bioengineer.org © Copyright 2023 All Rights Reserved.

No Result
View All Result
  • Homepages
    • Home Page 1
    • Home Page 2
  • News
  • National
  • Business
  • Health
  • Lifestyle
  • Science

Bioengineer.org © Copyright 2023 All Rights Reserved.

Welcome Back!

Login to your account below

Forgotten Password?

Retrieve your password

Please enter your username or email address to reset your password.

Log In